The formation of a blueshifted congener dye can obscure the multicolor fluorescence imaging, leading to misinterpretation of the data. We also show that the deletion of a two-methine unit from the polymethine chain, which results in the formation of blueshifted products, commonly occurs in other cyanine dyes, such as Alexa Fluor 647 (AF647) and Cyanine5.5. The carbonyl products generated from singlet oxygen-mediated photooxidation of Cy5 undergo a sequence of carbon–carbon bond-breaking and -forming events to bring about the novel dye-to-dye transformation. Our studies show that the formal C 2H 2 excision from Cy5 occurs mainly through an intermolecular pathway involving a combination of bond cleavage and reconstitution while unambiguously confirming the identity of the fluorescent photoproduct of Cy5 to be C圓 using various spectroscopic tools. Here, we report the mechanism for the photoconversion of Cy5 to C圓 that occurs upon photoexcitation during fluorescent imaging. However, recent observations that blueshifted derivatives of Cy dyes are formed via photoconversion have raised concerns as to the potential artifacts in multicolor imaging. Slim CI to the max: If you've only changed one column across a few models, rather than running the changed models and all their children, you'd only need to run + test downstream models that use the affected column.Cyanine (Cy) dyes are among the most useful organic fluorophores that have found a wide range of applications in single-molecule and super-resolution imaging as well as in other biophysical studies."dbt column advisor." If dbt has a full picture of how a column is produced-which input columns, which transformations-it could flag when there are potentially duplicative columns across models, and help avoid the repeating of business logic.Or, better yet, dbt would understand that the column has been tested upstream, has not changed, and so does not need to run the same tests again.Extending what I described above, if a column has not transformed from model X to model Y-no renames, no aggregations-dbt could natively inherit its properties, such as description + tests. If a source PII/PHI column is the indirect input to a column in a downstream model, being able to mark the latter model as sensitive. So I do find it useful to think concretely about the kinds of things we'd hope to enable here: If dbt could produce an EXPLAIN-style plan, of every single SQL function performed to produce a single column, that would be very cool, and also tricky to read and reason about as a human being. This is definitely on our minds it's still a ways away.Īs with any compelling feature, column-level lineage feels both immensely valuable and a bit vague. In a world where we had this, and built it into dbt, we'd also have an AST representation of every column name, from relation, and SQL function. To capture column-level lineage for real for real, we'd need a validating SQL grammar-same as would, incidentally, for a decent linter / auto-formatter ( Automatic formatter for SQL #2356). Over in Doc (and potentially, Test) Inheritance #2995, and dbt doc blocks #1158 before then, we've been discussing ways that YAML anchors make a version of this possible today, and how it could be better in the future (cross-file anchors, souped-up docs blocks, hierarchical properties-as-config). Today, dbt developers have to duplicate a lot of resource properties (descriptions, tags, meta, tests) across models, even when model Y is just select * from model X. I view column-level lineage as existing in two orders of complexity: Thanks for opening, I'm surprised there wasn't already an issue for this :) It's something we're hearing and talking about a lot these days.
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